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KMID : 0848120200450030077
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International Journal of Oral Biology
2020 Volume.45 No. 3 p.77 ~ p.83
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Practical considerations for the study of the oral microbiome
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Yu Yeun-I
Lee Seo-Young
Na Hee-Sam
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Abstract
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In the oral cavity, complex microbial community is shaped by various host and environmental factors. Extensive literature describing the oral microbiome in the context of oral health and disease is available. Advances in DNA sequencing technologies and data analysis have drastically improved the analysis of the oral microbiome. For microbiome study, bacterial 16S ribosomal RNA gene amplification and sequencing is often employed owing to the cost-effective and fast nature of the method. In this review, practical considerations for performing a microbiome study, including experimental design, molecular analysis technology, and general data analysis, will be discussed.
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KEYWORD
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Oral microbiome, 16S rDNA, Bacterial associations, High-throughput nucleotide sequencing
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